Illumina (Overview)
Sequence facility (i.e. Duke GSACR; UNC GCFR; UF ICBR)
Sample preparation
Soil DNA RNA extraction (i.e. MO-BIO PowerSoil DNA/RNA Kit)
Plant DNA/RNA extraction (i.e. RNeasy Plant mini kit; CTAB/LiCl method, Liao et al, 2014)
DNA/RNA quantification (i.e. Qubit Fluorometric quantification) & qualification (i.e. bioanalyzer; Fragment analyzer)
cDNA library (i.e. Poly-A enrichment, small RNA enrichment, bacterial RNA enrichment)
Lab published scripts used for Metatranscriptomic analysis: https://github.com/NFREC-Liao-Lab
Bioinformatics tools at Wiki (i.e. RNAseq)
Sequence facility (i.e. Duke GSACR; UNC GCFR; UF ICBR)
Sample preparation
Soil DNA RNA extraction (i.e. MO-BIO PowerSoil DNA/RNA Kit)
Plant DNA/RNA extraction (i.e. RNeasy Plant mini kit; CTAB/LiCl method, Liao et al, 2014)
DNA/RNA quantification (i.e. Qubit Fluorometric quantification) & qualification (i.e. bioanalyzer; Fragment analyzer)
cDNA library (i.e. Poly-A enrichment, small RNA enrichment, bacterial RNA enrichment)
Lab published scripts used for Metatranscriptomic analysis: https://github.com/NFREC-Liao-Lab
Bioinformatics tools at Wiki (i.e. RNAseq)